用16S rRNA基因的内切酶图谱快速鉴别几种对虾病原菌   总被引：4，自引：0，他引：4  

孔杰  刘萍  张岩  杨丛海  叶军  徐怀恕  郭华荣 《海洋与湖沼》1997,28(5):449-452

坎普氏弧菌是青岛海洋大学生物系微生物实验室于１９８９－１９９０年自对虾养殖场中国对虾红腿病心脏及血淋巴中分离并鉴定的菌株，副溶血菌和溶藻胶弧菌两菌株于１９９４年９月得自中国科学院微生物研究所，为建立快速、准确的中国对虾病原菌的诊断技术，根据几种细菌的１６ＳｒＲＮＡ基因的序列，设计并合成该基因的多聚酶反应的引物ＰＬ１和ＰＬ２。并用该对引物分别从坎普氏弧菌、副溶血弧菌和溶藻胶弧菌的ＤＮＡ要品中扩增出分  相似文献   

几株原甲藻核糖体大亚基RNA基因的部分克隆及序列分析   总被引：1，自引：0，他引：1       下载免费PDF全文

王波  米铁柱  吕颂辉  孙军  李荣秀  于志刚 《海洋学报》2007,29(1):120-126

对7株赤潮原甲藻28S rDNA 5'端部分序列进行扩增、克隆和序列测定,并从GeneBank上获取14个原甲藻28S rDNA序列,用NJ法和ME法构建了原甲藻属的系统树,并对序列进行分析.结果表明,7株原甲藻28S rDNA扩增序列长度为950~958 bp,通过NJ法和ME法构建的系统树完全一致.大部分分离自不同海域的同种原甲藻的序列高度保守,而不同种间在序列高变区却有较大的差异.但来自南海海域的海洋原甲藻(Prorocentrum micans)与分离自其他海域的株系序列差异较大,甚至超出了有些种间的差异.由28S rDNA高变区获得的序列,有望成为浮游植物特异性分子探针设计的良好靶区域.  相似文献   

Comparative research on karyotype in three species of Arcidae     

Zheng Jiasheng  Wang Meilin  Guo Danhong  Xu Ximin  Gao Qinglan 《海洋学报(英文版)》1996,15(4):529-538

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Annual Anthropogenic Carbon Transport into the North Pacific Intermediate Water through the Kuroshio/Oyashio Interfrontal Zone: An Estimation from CFCs Distribution     

Tsuneo Ono  Yutaka W. Watanabe  Katsuyuki Sasaki 《Journal of Oceanography》2000,56(6):675-689

Vertical distribution of anthropogenic carbon content of the water (exDIC) in the Oyashio area just outside of the Kuroshio/Oyashio Interfrontal Zone (K/O Zone) was estimated by the simple 1-D advection-diffusion model calibrated by the distribution of chlorofluorocarbons (CFCs). The average concentration of exDIC for = 26.60–27.00 is multiplied by the volume transport of Oyashio water into the North Pacific Intermediate Water (NPIW) to estimate the annual transport of exDIC into NPIW through K/O Zone. The estimated transport of exDIC was 0.018–0.020 GtC/y, which corresponds to 15% of the whole total exDIC accumulation in the temperate North Pacific. A simple assessment using the NPIW 1-box model indicates that the current study explains at least 70% of the total annual transport of exDIC into NPIW, and that small exDIC sources for NPIW still exists in addition to K/O Zone.  相似文献   

High Precision Geoid Models HKGEOID-2000 for Hong Kong and SZGEOID-2000 for Shenzhen, China     

Zhicai Luo  Jinsheng Ning  Yongqi Chen  Zhanji Yang 《Marine Geodesy》2005,28(2):191-200

High precision geoid models HKGEOID-2000 for Hong Kong and SZGEOID-2000 for Shenzhen, China, have been developed with a hybrid approach of so-called sequential processing, using high precision GPS/leveling data, land and sea gravity anomalies, and digital terrain models. These two local geoid models have the same 1-km resolution. The estimated accuracy (external accuracy) is better than 1.7 cm for HKGEOID-2000 and 1.4 cm for SZGEOID-2000. Some common areas are covered by HKGEOID-2000 and SZGEOID-2000. So these two geoid models, along with high quality GPS/leveling data collected on the overlapping areas, can be used to detect the systematic bias between HKGEOID-2000 and SZGEOID-2000, as well as the difference between Hong Kong Principal Datum and 1956 yellow sea height datum of China, yielding RMS errors of 1.011 m and 1,003 m, respectively. Moreover, HKGEOID-2000, along with GPS ellipsoidal heights, is employed to determine the errors of the “orthometric heights” from purely trigonometric heighting, yielding an RMS error of 0.102 m. The combination of SZGEOID-2000 and GPS ellipsoidal heights has been used to replace the traditional spirit leveling and mapping, called GPS mapping.  相似文献   

耳鲍(Haliotis asinina)核糖体小亚基(18S rRNA)编码基因的克隆与序列分析   总被引：1，自引：0，他引：1       下载免费PDF全文

黄勃  方再光  刘均玲  周智  王小兵 《海洋与湖沼》2007,38(3):212-216

采用分子生物学的方法,对南海不同海区的两个地理群体耳鲍(Haliotis asinina)18S rRNA基因全长进行了克隆和序列分析,并将耳鲍18S rRNA基因的序列与NCBI数据库中已收录鲍的18SrRNA基因进行了比较。结果发现,南海耳鲍核糖体18S rRNA基因与耳鲍H.asinina isolate H11核糖体18S rRNA基因序列的相同率高达98%;同一地理群体内耳鲍核糖体18S rRNA基因序列完全一致;不同地理群体间耳鲍核糖体18S rRNA基因在碱基组成上的相似率为99%,仅在某些位点处发生了碱基替换,即腺嘌呤(T)被鸟嘌呤(G)替换;同时,将这两个不同群体中耳鲍的18S rRNA基因与泰国耳鲍18S rRNA基因序列进行比较分析发现,它们之间也只是发生了碱基替换。  相似文献   

无舵翼水下机器人路径跟踪控制研究   总被引：1，自引：0，他引：1  

甘永  万磊  陆雯雯  庞永杰 《海洋工程》2007,25(1):70-75

针对无舵翼水下机器人的各种不同任务要求下的路径跟踪控制进行研究。通过模拟人的运动行为,建立了虚拟避碰声纳模型。根据地形跟踪的方法提出基于虚拟声纳的路径跟踪控制方法,并通过考虑纵向速度对于其他各个自由度运动的影响设计了运动控制器。通过海上试验验证了所提出的路径跟踪控制方法对于无舵翼水下机器人是可以满足实际需要的。  相似文献   

Effect of strong winds on the nutritional condition of anchovy (Engraulis encrasicolus L.) larvae in the Bay of Biscay, Northeast Atlantic, as inferred from an early field application of the DNA/C index     

Bergeron  Jean-Pierre 《ICES Journal of Marine Science》2000,57(2):249-255

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Observations of the Labrador Sea eddy field   总被引：2，自引：0，他引：2  

Jonathan M. Lilly  Peter B. RhinesFriedrich Schott  Kara LavenderJohn Lazier  Uwe SendEric D’Asaro 《Progress in Oceanography》2003,59(1):75-176

This paper is an observational study of small-scale coherent eddies in the Labrador Sea, a region of dense water formation thought to be of considerable importance to the North Atlantic overturning circulation. Numerical studies of deep convection emphasize coherent eddies as a mechanism for the lateral transport of heat, yet their small size has hindered observational progress. A large part of this paper is therefore devoted to developing new methods for identifying and describing coherent eddies in two observational platforms, current meter moorings and satellite altimetry. Details of the current and water mass structure of individual eddy events, as they are swept past by an advecting flow, can then be extracted from the mooring data. A transition is seen during mid-1997, with long-lived boundary current eddies dominating the central Labrador Sea year-round after this time, and convectively formed eddies similar to those seen in deep convection modeling studies apparent prior to this time. The TOPEX / Poseidon altimeter covers the Labrador Sea with a loose “net” of observations, through which coherent eddies can seem to appear and disappear. By concentrating on locating and describing anomalous events in individual altimeter tracks, a portrait of the spatial and temporal variability of the underlying eddy field can be constructed. The altimeter results reveal an annual “pulsation” of energy and of coherent eddies originating during the late fall at a particular location in the boundary current, pinpointing the time and place of the boundary current-type eddy formation. The interannual variability seen at the mooring is reproduced, but the mooring site is found to be within a localized region of greatly enhanced eddy activity. Notably lacking in both the annual cycle and interannual variability is a clear relationship between the eddies or eddy energy and the intensity of wintertime cooling. These eddy observations, as well as hydrographic evidence, suggest an active role for boundary current dynamics in shaping the energetics and water mass properties of the interior region.  相似文献   

Geographic variability in trace element levels in Spartina alterniflora     

J.E. Drifmeyer  B. Redd 《Estuarine, Coastal and Shelf Science》1981,13(6):709-716

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